FLS

Arabidopsis thaliana

Brassicaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

dihydrokaempferol

PubChem ↗ C1=CC(=CC=C1[C@@H]2[C@H](C(=O)C3=C(C=C(C=C3O2)O)O)O)O

FLS

ΔMass: -2.0157 Da ketone_formation(-2.0157)

Product

kaempferol

PubChem ↗ C1=CC(=CC=C1C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)O)O

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; FLS from Arabidopsis thaliana (AT5G08640) expressed in Yarrowia lipolytica; conversion of dihydrokaempferol to kaempferol confirmed by HPLC and LC-MS.

Reaction & quality gates

Mechanism: Ketone formation (-2.02 Da)
Evidence type: In vivo
Confidence: High
Data tier: Quarantine
non-CYP auxiliary enzyme (pathway partner, not cytochrome P450)
Substrate class: Phenylpropanoids
Product class: Phenylpropanoids

Literature & public identifiers

PubMed: PMID:39841399 ↗
DOI: 10.1186/s40643-024-00825-w ↗
UniProt: Q9XHG2 ↗
GenBank: —
NCBI TaxID: 3702 ↗

De novo biosynthesis of quercetin in Yarrowia Lipolytica through systematic metabolic engineering for enhanced yield.