CYP94D65

Avena strigosa CYP94D

Poaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

β-amyrin

PubChem ↗ [H][C@]12[C@H](O)C=C3[C@]4([H])CC(C)(C)CC[C@]4(C)CC[C@@]3(C)[C@…

CYP94D65

ΔMass: no_smiles Missing dual SMILES or RDKit not validated

Product

23-hydroxy-β-amyrin

PubChem ↗ Crowdsource product structure

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; CYP94D65 adds a hydroxyl group at C-23 position of β-amyrin scaffold; identified via transient expression in Nicotiana benthamiana and mutant #1303 (G1385A mutation causing premature termination).

Reaction & quality gates

Mechanism: Hydroxylation
Evidence type: In vivo
Confidence: High
Data tier: Silver
mass=no_smiles; incomplete_SMILES
Substrate class: Terpenoids
Product class: Terpenoids

Literature & public identifiers

PubMed: PMID:40106352 ↗
DOI: 10.1073/pnas.2417588122 ↗
UniProt: XRL31189.1 ↗
GenBank: CYP94D65 ↗
NCBI TaxID: 38783 ↗

Comprehensive mutant chemotyping reveals embedding of a lineage-specific biosynthetic gene cluster in wider plant metabolism.