C3H

Metabolic engineering

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

p-coumaric acid

PubChem ↗ C1=CC(=CC=C1/C=C/C(=O)O)O

C3H

ΔMass: +15.9949 Da hydroxylation(+15.9949)

Product

caffeic acid

PubChem ↗ C1=CC(=C(C=C1/C=C/C(=O)O)O)O

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; Coumarate-3-hydroxylase from Populus trichocarpa; CA was undetectable in HYC21, suggesting low activity.

Reaction & quality gates

Mechanism: Hydroxylation (+15.99 Da)
Evidence type: In vivo
Confidence: High
Data tier: Gold
Latin binomial + experiment + dual SMILES + ΔMass pass
Substrate class: Phenylpropanoids
Product class: Phenylpropanoids

Literature & public identifiers

PubMed: PMID:40756073 ↗
DOI: 10.1016/j.synbio.2025.07.005 ↗
UniProt: EU603301 ↗
GenBank: EU603301 ↗
NCBI TaxID: —

Metabolic engineering <i>Saccharomyces cerevisiae</i> for <i>de novo</i> biosynthesis of hydroxycinnamoyl glycerols.