CYP82D274

Tripterygium wilfordii CYP82D

Celastraceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

miltiradiene

PubChem ↗ CC(C)C1=CCC2=C(C1)CC[C@@H]3[C@@]2(CCCC3(C)C)C

CYP82D274

ΔMass: no_smiles Missing dual SMILES or RDKit not validated

Product

14-hydroxy-dehydroabietadiene

PubChem ↗ Crowdsource product structure

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; CYP82D274 catalyzes C-14 hydroxylation of miltiradiene to form 14-hydroxy-dehydroabietadiene, validated in engineered S. cerevisiae (WAT11 and INVsc1) and N. benthamiana. Source organism is Tripterygium wilfordii based on gene origin.

Reaction & quality gates

Mechanism: Hydroxylation
Evidence type: In vivo
Confidence: High
Data tier: Silver
mass=no_smiles; incomplete_SMILES
Substrate class: Terpenoids
Product class: Other

Literature & public identifiers

PubMed: PMID:41555396 ↗
DOI: 10.1186/s12934-025-02917-4 ↗
UniProt: —
GenBank: —
NCBI TaxID: 458696 ↗

Efficient biosynthesis of the plant-derived diterpenoid 14-hydroxy-dehydroabietadiene in Saccharomyces cerevisiae.