CYP85A2

Arabidopsis thaliana CYP85A

Brassicaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

castasterone

PubChem ↗ C[C@@H]([C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC(=O)[C@@H]4[C@@]3…

CYP85A2

ΔMass: +15.9949 Da hydroxylation(+15.9949)

Product

brassinolide

PubChem ↗ C[C@@H]([C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2COC(=O)[C@@H]4[C@@]…

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; Crude enzyme from seeds of transgenic Brachypodium expressing AtCYP85A2 catalyzed conversion of CS to BL; BL synthase activity confirmed.

Reaction & quality gates

Mechanism: Hydroxylation (+15.99 Da)
Evidence type: In vitro
Confidence: High
Data tier: Gold
Latin binomial + experiment + dual SMILES + ΔMass pass
Substrate class: Other
Product class: Other

Literature & public identifiers

PubMed: PMID:33868337 ↗
DOI: 10.3389/fpls.2021.639508 ↗
UniProt: Q940V4 ↗
GenBank: —
NCBI TaxID: 3702 ↗

Seed-Specific Expression of <i>Arabidopsis AtCYP85A2</i> Produces Biologically Active Brassinosteroids Such as Castasterone and Brassinolide to Improve Grain Yield and Quality in Seeds of <i>Brachypodium Distachyon</i>.

Evidence trail

Reaction & quality gates

Literature & public identifiers

Other reactions by this enzyme