CYP74

Linum usitatissimum CYP74

Linaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

13(S)-hydroperoxylinolenic acid

PubChem ↗ CC/C=C\C[C@@H](/C=C/C=C\CCCCCCCC(=O)O)OO

CYP74

ΔMass: Exempt atypical_cleavage_exempt

Product

allene oxide

PubChem ↗ C=C1CO1

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt Other; Other; Flaxseed allene oxide synthase (CYP74) converts 13(S)-hydroperoxylinolenic acid to allene oxide, a precursor of jasmonic acid. Evidence from purified enzyme and recombinant expression in HEK 293 cells.

Reaction & quality gates

Mechanism: Atypical_Cleavage (Hydroperoxide lyase)
Evidence type: In vitro
Confidence: High
Data tier: Special
Atypical P450 cleavage (CYP74); ΔMass exempt
Substrate class: Organic acids
Product class: Other

Literature & public identifiers

PubMed: PMID:8378325 ↗
DOI: 10.1073/pnas.90.18.8519 ↗
UniProt: P48417 ↗
GenBank: —
NCBI TaxID: 4006 ↗

Molecular cloning of an allene oxide synthase: a cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides.