CYP749B1

Withania somnifera CYP749B

Solanaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

12-deoxywithastramonolide

PubChem ↗ CC1=C(C(=O)O[C@H](C1)[C@@H](C)[C@H]2CC[C@@H]3[C@@]2(CC[C@H]4[C@…

CYP749B1

ΔMass: Exempt atypical_cleavage_exempt

Product

withanolide A

PubChem ↗ CC1=C(C(=O)O[C@H](C1)[C@@](C)([C@H]2CC[C@@H]3[C@@]2(CC[C@H]4[C@…

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; Overexpression of WsCYP749B1 increased withanolide A (>250%) and decreased 12-deoxywithastramonolide (~60%), suggesting conversion.

Reaction & quality gates

Mechanism: Atypical_Cleavage (Hydroperoxide lyase)
Evidence type: In vivo
Confidence: High
Data tier: Special
Atypical P450 cleavage (CYP74); ΔMass exempt
Substrate class: Other
Product class: Other

Literature & public identifiers

PubMed: PMID:35102209 ↗
DOI: 10.1038/s41598-022-05634-9 ↗
UniProt: B3RFJ8 ↗
GenBank: —
NCBI TaxID: 126910 ↗

Molecular characterization of three CYP450 genes reveals their role in withanolides formation and defense in Withania somnifera, the Indian Ginseng.

Evidence trail

Reaction & quality gates

Literature & public identifiers

Other reactions by this enzyme