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Gold ΔMass ✓ Hydroxylation

HPO

Hyoscyamus muticus CYP71D

Solanaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
premnaspirodiene
PubChem ↗ C[C@@H]1CCC=C([C@]12CC[C@H](C2)C(=C)C)C
HPO
ΔMass: +15.9949 Da hydroxylation(+15.9949)
Product
solavetivol
PubChem ↗ C[C@@H]1C[C@@H](C=C([C@]12CC[C@H](C2)C(=C)C)C)O

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; HPO catalyzes regio- and stereo-specific hydroxylation at C-2 of premnaspirodiene to form solavetivol; evidence from yeast microsome assays with NADPH.

Reaction & quality gates

Mechanism
Hydroxylation (+15.99 Da)
Evidence type
In vitro
Confidence
High
Data tier
Gold
Latin binomial + experiment + dual SMILES + ΔMass pass
Substrate class
Other
Product class
Other

Literature & public identifiers

PubMed
PMID:17715131 ↗
DOI
10.1074/jbc.m703378200 ↗
UniProt
A6YIH8 ↗
GenBank
NCBI TaxID
35626 ↗

Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates.